牛乳源金黄色葡萄球菌黏附素分子FnBPA不同功能区生物学活性的比较分析

doi:10.11843/j.issn.0366-6964.2015.07.018

Abstract

Abstract:

The aim of this study was to express FnBPA-A（A domain of fibrinogen-binding region A）and FnBPA-BCD (BCD domain of fibrinogen-binding region A different domains of FnBPA) of Staphylococcus aureus，and compare the adhesion inhibition activities of their antiserum for prevention of bovine mastitis caused by S.aureus.FnBPA-A and FnBPA-BCD encoding genes were cloned and inserted into pET-28a vector.Then the recombinant proteins were expressed and purified to immune Newzeland rabbits and mice.The adhesin binding ability，adhesion inhibition activity of their serum antibody and challenge protection ability were tested.The results showed that the antibody level，the binding ability to Fg and Fn and adhesion inhibition activity of FnBPA-A and FnBPA-BCD co-immunization group were better than that of single immunization groups.The mice antibody level of FnBPA-A immunization group and co-immunization group were better than that of FnBPA-BCD immunization group.In addition，FnBPA-A and co-immunization group showed better adhesion inhibition activity to Fg，FnBPA-BCD and co-immunization group showed better adhesion inhibition activity to Fn.The immunological protection result showed that each group had good protective effect and FnBPA-A immunization group was the best one.The results of this study may provide the basis for design of new vaccines of bovine mastitis caused by S.aureus.

CLC Number:

S852.611

SHAO Jun-gao，ZHANG Bao-jiang，LI Shan-chun，WANG Cai-die，WANG Guo-qin，SU Yan. Comparison and Analysis of the Biological Activity of Antiserum after Immunizing with Expressed Different Domains of Staphylococus aureus Adhesin FnBPA Isolated from Bovine Milk[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1208-1214.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.07.018

https://www.xmsyxb.com/EN/Y2015/V46/I7/1208

References

［1］LAMMERS A，NUIJTEN P J，KRUIJT E，et al.Cell tropism of Staphylococcus aureus in bovine mammary gland cell cultures［J］.Vet Microbiol，1999，67(2)：77-89.
［2］陈怀青，陆承平.金黄色葡萄球菌的粘附素［J］.中国人兽共患病杂志，1996，12(6)：43-44.
CHEN H Q，LU C P.The adhesins of Staphylococcus aureus［J］.Chinese Journal of Zoonoses，1996，12(6)：43-44.(in Chinese)
［3］周宏，李韩平，姜永强，等.金黄色葡萄球菌表面蛋白研究进展［J］.生物技术通讯，2004，15(1)：73-75.
ZHOU H，LI H P，JIANG Y Q，et al.The progress of Staphylococcus aureus surface proteins［J］. Letters in Biotechnology，2004，15(1)：73-75.(in Chinese)
［4］任士飞，徐建生，董国雄，等.细菌黏附研究进展［J］.中国预防兽医学报，2004，26(3)：238-240.
REN S F，XU J S，DONG G X，et al.Progress in research of bacterial adherence［J］.Chinese Journal of Preventive Veterinary Medicine，2004，26(3)：238-240.(in Chinese)
［5］KEANE F M，LOUGHMAN A，VALTULINA V，et al.Fibrinogen and elastin bind to the same region within the A domain of fibronection binding protein A，an MSCRAMM of Staphylococcus aureus［J］.Mol Microbiol，2007，63(3)：711-723.
［6］LOUGHMAN A，SWEENEY T，KEANE F M，et al.Sequence diversity in the A domain of Staphylococcus aureus fibronectin-binding protein A［J］.BMC Microbiol，2008，8：74.
［7］CASOLINI F，VISAI L，JOH D，et al.Antibody response to fibronectin-binding adhesin FnbpA in patients with Staphylococcus aureus infections［J］.Infect Immun，1998，66(11)：5433-5442.
［8］JOH H J，HOURSE P K，PATTI J M，et al.Fibronectin receptors from Gram-positive bacteria：comparison of active sites［J］.Biochemistry，1994，33(20)：6086-6092.
［9］何焱，谢红霞，杨岚，等.牛源金黄色葡萄球菌FnBPA A基因与D基因表达产物的免疫原性比较［J］.中国兽医科学，2014，44(5)：480-484.
HE Y，XIE H X，YANG L，et al.Comparison of immunogenicity between the expressed proteins of FnBP A gene and D gene of Staphylococcus aureus from cattle［J］.Chinese Veterinary Science，2014，44(5)：480-484.(in Chinese)
［10］杨宏军，高运东，王长法，等.金黄色葡萄球菌粘附素Fnbp A功能区基因的克隆和原核表达［J］.西北农林科技大学学报：自然科学版，2008，36(1)：54-58.
YANG H J，GAO Y D，WANG C F，et al.Cloning and expression of the functional Fnbp A gene of Staphylococcus aureus adhesin［J］.Journal of Northwest A&F University(Natural Science Edition)，2008，36(1)：54-58.(in Chinese)
［11］赵艳，张爱荣，郝永清.金黄色葡萄球菌的FnBPA D基因片段的表达［J］.中国预防兽医学报，2009，31(5)：361-365.
ZHAO Y，ZHANG A R，HAO Y Q.Cloning and expression of the D fragment of fibronectin-binding protein A gene from Staphylococcus aureus［J］.Chinese Journal of Preventive Veterinary Medicine，2009，31(5)：361-365.(in Chinese)
［12］BROUILLETTE E，LACASSE P，SHKRETA L，et al.DNA immunization against the clumping factor A (ClfA) of Staphylococcus aureus［J］.Vaccine，2002，20(17-18)：2348-2357.
［13］史冬艳，郝永清，张爱荣.奶牛乳房炎金黄色葡萄球菌FnbpB-D基因的表达及其抗血清活性［J］.中国预防兽医学报，2010，32(5)：356-359.
SHI D Y，HAO Y Q，ZHANG A R.Expression of fibronectin-binding protein B (FnbpB) region D of Staphylococcus aureus and antiserum activity［J］.Chinese Journal of Preventive Veterinary Medicine，2010，32(5)：356-359.(in Chinese)
［14］张向新，石庆华，王世民，等.新疆奶牛乳腺炎致病性金黄色葡萄球菌FnbpA 基因的克隆与序列分析［J］.新疆农业大学学报，2010，33(5)：422-426.
ZHANG X X，SHI Q H，WANG S M，et al.Clone and sequence analysis of FnbpA gene of Staphylococcus aureus isolated from Xinjiang dairy cow with subclinical Mastitis［J］.Journal of Xinjiang Agricultural University，2010，33(5)：422-426.(in Chinese)
［15］范鑫，郝永清，张爱荣，等.金黄色葡萄球菌黏附素FnBPB-ClFA共表达质粒的构建与原核表达［J］.中国兽医科学，2011，41(2)：173-177.
FAN X，HAO Y Q，ZHANG A R，et al.Construction and prokaryotic expression of the co-expression plasmid with FnBPB-ClFA gene encoding Staphylococcus aureus adhesion［J］.Chinese Veterinary Science，2011，41(2)：173-177.(in Chinese)
［16］GAUDREAU M C，LACASSE P，TALBOT B G.Protective immune responses to a multi-gene DNA vaccine against Staphylococcus aureus［J］.Vaccine，2007，25(5)：814-824.

[1]	Lü Ruoyi, SI Xiaohui, SUN Zhigang, SHI Xiaomin, LIU Xiaoye. Drug Resistance Situation of Streptococcus suis and Prevention Measures of Infections [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4920-4933.
[2]	HU Xiuhua, SUN Zhixin, ZHAO Mengyang, XIE Jiaqi, WANG Min, CHEN Hailiang, GE Xin, LIU Tianlong, WANG Shaolin. Pathogenicity and Resistance Analysis of Enterococcus faecium from Wild Squirrels [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3012-3021.
[3]	LU Wanqing, ZHAO Shasha, JIANG Songhong, TONG Zhizi, HUANG Danni, GUO Jianhua, WU Junwei, ZHOU Yang. Effect of Staphylococcus aureus on IFN-α Production in BV2 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2633-2641.
[4]	MAO Yanni, CHANG Jiawei, LI Na, WANG Xin, KANG Xinyun, MA Qiang, MA Liang, WANG Guiqin. Transcriptome Differential Expression Analysis of Staphylococcus aureus in Biofilm State and Planktonic State [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2697-2707.
[5]	DU Yu, PU Xiaofeng, CHEN Xiaomeng, Lü Fenfen, WANG Li, ZHANG Zehua, ZHANG Baojiang, SU Yan. Identification and Evolutionary Analysis of Three Novel SeM Genotypes of Donkey Streptococcus equi subsp. equi Isolates in Xinjiang [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 231-240.
[6]	ZHANG Haixia, ZHANG Zhenbiao, DAI Hegen, SONG Yu, LI Lei, XIA Zhaofei. Analysis of Epidemiological Characteristics of Clinical Staphylococcus pseudintermedius Isolated from Dogs [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2561-2568.
[7]	ZHANG Jing, CAI Ping, HE Yizhuo, CHEN Weitao, HUANG Yuchen, JIANG Hongxia. Prevalence of Antimicrobial Resistance and Virulence Factors in Enterococci from Poultry Farms in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2650-2659.
[8]	ZHANG Jinning, QIAN Mengying, TANG Yongjie, MI Siyuan, SHI Kerong, YU Ying. The Adhesion Effect of Staphylococcus aureus Surface Protein A on Dairy Cattle Mammary Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1369-1377.
[9]	LÜ Fenfen, MA Xiaohui, WANG Li, MA Chen, ZHANG Baojiang, SU Yan. Immunogenicity Analysis of Three Different Antigen Proteins of Streptococcus equi in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 752-762.
[10]	ZHANG Hang, LI Xinpu, WU Xiaohu, DING Xuezhi, YAN Zuoting, WANG Shengyi, LI Hongsheng. Investigation of Biofilm Formation, Virulence Genes and agr Typing of Staphylococcus aureus from Bovine Mastitis Cases [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2903-2910.
[11]	MA Qiang, YANG Rui, WAN Jiahong, CHANG Jiawei, WEI Yanqin, WANG Guiqin. Study on β-lactamase and Its Action Mode of Staphylococcus aureus Isolated from Cows in Ningxia [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1138-1148.
[12]	MA Qiang, WANG Yihui, CHANG Jiawei, WAN Jiahong, WEI Yanqin, WANG Guiqin. The Relationship between Cell Wall Thickening of Staphylococcus aureus and Resistance to β-lactams [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 602-611.
[13]	MA Weiwu, ZHOU Xuezhang. Expression of Panton-Valenine Leukocidin from Staphylococcus aureus and Its Effects on Bovine Mammary Epithelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2105-2112.
[14]	GONG Yongping, CHEN Zhenrong, YIN Wenqi, WEN Jifeng, YI Keke, YAN Qigui. Analysis of Multidrug-resistant Genomic Island PRI1 of Enterococcus faecium LS170308 from Moschus berezovskii [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1275-1283.
[15]	CHEN Shenshen, MA Jiale, ZHANG Xinfeng, QU Xiangyang, PAN Zihao, YAO Huochun. Isolation and Identification of Streptococcus suis Causing Meningitis in Piglets [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 143-150.

Comparison and Analysis of the Biological Activity of Antiserum after Immunizing with Expressed Different Domains of Staphylococus aureus Adhesin FnBPA Isolated from Bovine Milk

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments